WSSA Meeting Abstracts, Crystallographic snapshot of cellulose synthesis and membrane translocation, Visualization of cellulose synthase demonstrates functional association with microtubules, Genetic evidence for three unique components in primary cell-wall cellulose synthase complexes in, -glucanase involved in cellulose synthesis undergoes regulated intracellular cycling, Herbicides that inhibit cellulose biosynthesis, Modifications of cellulose synthase confer resistance to isoxaben and thiazolidinone herbicides in, Tertiary model of a plant cellulose synthase, The sites of cellulose synthesis in algae: diversity and evolution of cellulose-synthesizing enzyme complexes, Semimicro determination of cellulose in biological materials, Chemical genetic screening identifies a novel inhibitor of parallel alignment of cortical microtubules and cellulose microfibrils, RBR-Type E3 Ligases and the Ubiquitin-Conjugating Enzyme UBC26 Regulate Abscisic Acid Receptor Levels and Signaling, CLE40 Signaling Regulates Root Stem Cell Fate, Design Principle for Decoding Calcium Signals to Generate Specific Gene Expression Via Transcription, by The American Society of Plant Biologists, http://wssa.net/meeting/meeting-abstracts. In turf, C4 warm-season turfgrass species generally have good tolerance to indaziflam. The average colocalization rate over three experimental runs (total n = 544) between YFP:CESA6 particles and RFP:TUA5 after indaziflam treatment was 53% ± 4%. 2,6-Dimethyl-1-indanon wird katalysiert durch einen Ruthenium(II)-Komplex stereospezifisch zu cis-2,6-Dimethyl-indan-1-ol reduziert. Indaziflam Herbicidal Action: A Potent Cellulose Biosynthesis Inhibitor, Quantification of colocalization between CESA and, Thaxtomin A affects CESA-complex density, expression of cell wall genes, cell wall composition, and causes ectopic lignification in, Chemical genetics to examine cellulose biosynthesis, POM-POM2/cellulose synthase interacting1 is essential for the functional association of cellulose synthase and microtubules in, Smooth crabgrass control with indaziflam at various springs timings, Pausing of Golgi bodies on microtubules regulates secretion of cellulose synthase complexes in, Random GFP:cDNA fusions enable visualization of subcellular structures in cells of, Combining polysaccharide biosynthesis and transport in a single enzyme: dual-function cell wall glycan synthases, Morlin, an inhibitor of cortical microtubule dynamics and cellulose synthase movement, Nonmotile cellulose synthase subunits repeatedly accumulate within localized regions at the plasma membrane in Arabidopsis hypocotyl cells following 2,6-dichlorobenzonitrile treatment, Cellulose biosynthesis: exciting times for a difficult field of study, Organization of cellulose synthase complexes involved in primary cell wall synthesis in, Resistance against herbicide isoxaben and cellulose deficiency caused by distinct mutations in same cellulose synthase isoform CESA6, The cellulose synthase complex: a polymerization driven supramolecular motor, Spatial relationship between microtubules and plasma-membrane rosettes during the deposition of primary wall microfibrils in, Identification of a cellulose synthase-associated protein required for cellulose biosynthesis, Cellulose microfibril crystallinity is reduced by mutating C-terminal transmembrane region residues CESA1A903V and CESA3T942I of cellulose synthase, The international survey of herbicide resistant weeds, Isoxaben inhibits the synthesis of acid insoluble cell wall materials in, Effects of 2,6-DCB on plasma membrane rosettes of wheat root cells, Occurrence of the putative cellulose-synthesizing “Rosettes” in the plasma membrane of, Cellulose biosynthesis: counting the chains, Utilizing R software package for dose-response studies: the concept and data analysis, In vitro versus in vivo cellulose microfibrils from plant primary wall synthases: structural differences, Cellulose synthase interactive protein 1 (CSI1) mediates the intimate relationship between cellulose microfibrils and cortical microtubules, Cellulose synthase interactive protein 1 (CSI1) links microtubules and cellulose synthase complexes, The phenolic profile of maize primary cell wall changes in cellulose-deficient cell cultures, Indaziflam/BCS-AA10717-A new herbicide for pre-emergent control of grasses and broadleaf weeds for turf and ornamentals. It also controls annual sedges and broadleaf weeds in turf, ornamentals (residential), hedgerows, hardscapes, Christmas trees, and conifers. All Arabidopsis (Arabidopsis thaliana) seedlings were grown vertically on one-half-strength Murashige and Skoog (MS) Basal Salt Mixture (PhytoTechnology Laboratories) agar plates under continuous light or dark conditions. The material was then boiled in nitric acid-acetic acid for 30 min, cooled, and centrifuged for 5 min to pelletize insoluble material. Indaziflam is labeled for use in turf, for perennial crops, and for nonagricultural situations for preemergent control of grasses and broadleaf weeds (Meyer et al., 2009; Brosnan et al., 2011). The following materials are available in the online version of this article. [See online article for color version of this figure.]. Supplemental Movie S1. The coincidence between PM CESA particles and MTs is normally around 70% to 80% (Paredez et al., 2006; Li et al., 2012). Asterisks indicate a significant difference (n = 60; P < 0.05) in the GR50 values between the mutant and the wild type. 1121375 to Y.G. This was repeated thrice to remove any remaining [14C]Glc in solution. Indaziflam also caused reduced CESA particle velocity and partial uncoupling from the MT array. Here, we characterize the herbicide indaziflam as a CBI and provide insight into its inhibitory mechanism. Imaging was performed on a Yokogawa CSUX1 spinning disk system featuring a DMI6000 Leica motorized microscope, a Photometrics QuantEM:512SC CCD camera, and a Leica 100×/1.4 numerical aperture oil objective. Indaziflam caused CBI symptomologies, including radial swelling and ectopic lignification, in both Arabidopsis and P. annua treated seedlings (Fig. Indaziflam is currently at an advanced stage of development. A total of 20 hypocotyl or root lengths from each Arabidopsis line and 12 P. annua roots were measured 7 d after treatment. Indaziflam is one of ten new active ingredients that Bayer CropScience is planning to launch between 2008 and 2012, which together have a peak sales potential in excess of EUR 1 billion. Es wurde 2010 von Bayer CropScience in den Vereinigten Staaten auf den Markt gebracht. and Plant and Soil Science (M.B. For live-cell imaging, 3-d-old dark-grown seedlings expressing YFP:CESA6 (Paredez et al., 2006) or YFP:CESA6- RFP:TUA5 (Gutierrez et al., 2009) were used. Next, 0.5 mL of Glc-free MS media solution containing [14C]Glc at 1 μCi mL−1 was added to each tube followed by the addition of treatments. Error bars are ±1 se from the mean. The loss of function in any of the aforementioned proteins causes complete or partial loss of anisotropic growth in cells undergoing expansion, resulting in radial swelling. Thank you for your interest in spreading the word on Plant Physiology. 1A), an herbicide introduced by Bayer Crop Science, was recently proposed to be a CBI and was reported to have a photosystem II inhibition value of 9.4 (Meyer et al., 2009; Dietrich and Laber, 2012). Indaziflam treatment comprised 500 nm indaziflam for 2 h. CSI1 was identified as a primary linker protein between MTs and CSCs (Gu et al., 2010; Bringmann et al., 2012; Lei et al., 2012; Li et al., 2012). Indaziflam caused neurobehavioral effects and/or neuropathological changes in subchronic studies in rats and dogs. Single optical sections (monochrome) show the distribution of YFP:CESA6-labeled puncta upon 2-h 0.01% DMSO mock treatment (left) or 500 nm indaziflam treatment (right). Already Headed for Dicamba-Resistant Weeds? 4B). One measurement is the velocity (positional movement) of PM-localized CESA particles. Visually, indaziflam-treated seedlings exhibited radial swelling (Fig. B, The histogram depicts the frequency of YFP:CESA6 particle velocities at the PM focal plane after a 2-h treatment with indaziflam or DMSO mock. Classification of an herbicide to the CBI designation was traditionally achieved by short-term [14C]radioisotope tracer studies focused on the incorporation of Glc into cellulose (Heim et al., 1990; Sabba and Vaughn, 1999). In einer Studie wurde herausgefunden, dass Pflanzen mit Resistenzen gegen andere Cellulose-Biosynthese-Hemmer wie beispielsweise Isoxaben nicht gegen Indaziflam resistent sind.  Now registered for general turf situations. This makes indaziflam an environmentally compatible and pioneering … Here, the behavior of YFP:CESA6 in indaziflam-treated cells resembled the behavior of CESAs in the CSC-MT linker protein, csi1, mutant background (Gu et al., 2010). Indaziflam is an excellent mixing partner and can be used either pre- or post-emergent in conjunction with post-emergent herbicides. Seedlings were treated for 1 h in the dark with either DMSO (0.01% [v/v]) or indaziflam (32 nm). Es sind folglich acht verschiedene Isomere möglich. A, Chemical structure of indaziflam. The principle catalytic unit is a plasma membrane (PM)-localized protein complex referred to as the cellulose synthase complex (CSC; Davis, 2012). Analysis of time-lapse images from seedlings in the absence of indaziflam revealed a dynamic population of YFP:CESA6-labeled particles residing at the PM (Supplemental Movie S1). Treatments were indaziflam at 0, 200, or 400 pm. Indaziflam is an excellent mixing partner and can be used either pre- or post-emergent in conjunction with post-emergent herbicides. For lignin staining, 7-d-old light-grown seedlings were incubated in ethanol (70%) for 24 h followed by 30 min in a 2% (w/v) phloroglucinol solution (20% hydrochloric acid). Rather, the movement of CESA particles was proposed to be a function of a polymerization force generated by the translocating glucan chain (Diotallevi and Mulder, 2007). However, these same mutants have a 300-fold and 90-fold level of resistance to isoxaben, respectively (Heim et al., 1989). Cellulose content in the hypocotyl region of 5-d-old dark-grown Arabidopsis seedlings was determined by boiling 5 mg dry weight of the plant in nitric acid-acetic acid (Updegraff, 1969). Indaziflam is a selective herbicide providing pre-emergence and post- emergence (when indaziflam is formulated with 2,4-D, dicamba, mecoprop, and penoxsulam) control of … (2007b) found that the majority of the accumulated PM-localized YFP:CESA6 particles did not exhibit detectable movement 1 h after treatment (maximum velocity 34 nm min−1). Indaziflam effects also share little similarity with those caused by DCB. S1). If resistance is managed, indaziflam has the potential to be a valuable alternative mode of action for weed management. The recently introduced herbicide indaziflam has a mode of action not previously used in non-crop weed management.

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